中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (39): 6335-6341.doi: 10.3969/j.issn.2095-4344.2014.39.018

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

钛基表面纳米银复合涂层的缓释性能

鲜爱明，张晓岗，曹  力，王佳明，彭理斌，胡  洋   

  1. 新疆医科大学第一附属医院骨科中心，新疆维吾尔自治区乌鲁木齐市  830054
  • 出版日期:2014-09-17 发布日期:2014-09-17
  • 通讯作者: 曹力，教授，主任医师，博士研究生导师，新疆医科大学第一附属医院骨科中心主任，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:鲜爱明，男，1975年生，四川省南充市人，汉族，主治医师，新疆医科大学在读硕士，主要从事骨与关节疾病方面的研究。
  • 基金资助:

    新疆维吾尔自治区自然科学基金(2013211A075)，项目名称：纳米银/羟基磷灰石(Ag-HA)纳米复合涂层抗菌性能的体内体外试验研究

Release properties of titanium-based nano-silver coating

Xian Ai-ming, Zhang Xiao-gang, Cao Li, Wang Jia-ming, Peng Li-bin, Hu Yang   

  1. Department of Orthopaedic Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Online:2014-09-17 Published:2014-09-17
  • Contact: Cao Li, Professor, Chief physician, Doctoral supervisor, Department of Orthopaedic Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Xian Ai-ming, Studying for master’s degree, Attending physician, Department of Orthopaedic Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2013211A075

PDF

719

被引次数

4

摘要：

背景：骨科植入材料表面改性可以减少或避免细菌黏附，利用金属表面的特殊涂层释放抑菌及杀菌成分可以预防骨科术后感染。
目的：选择不同的制备参数，在医用纯钛表面沉积羟基磷灰石/纳米银复合涂层，观测复合材料表面银离子在模拟体液中的缓释性能。
方法：利用脉冲电化学方法，在含银、钙、磷离子的溶液中实现纳米银和羟基磷灰石在钛表面的共沉积，再高温烧结处理制得复合涂层钛材料，用扫描电镜、X射线衍射仪、能谱仪对其形貌、组成进行表征。将分别含有0.5，1 mmol/L银的复合涂层钛材料浸泡在模拟体液中，在不同的时间点用原子能吸收光谱法检测Ag+浓度。
结果与结论：复合涂层是由纳米针状羟基磷灰石和点状的银颗粒相互交织成的网状结构，纳米银颗粒在涂层中均匀分布。高温处理后涂层变得致密，羟基磷灰石的晶化度提高，银颗粒在烧结后无团聚现象。在模拟体液中，第1-7天Ag+释放量最大，第7-30天Ag+释放量趋于稳定并维持有效抗菌浓度。含0.5 mmol/L银复合涂层钛材料在30 d时Ag+释放总量低于细胞毒性浓度，含1.0 mmol/L银复合涂层钛材料在30 d时Ag+释放总量接近细胞毒性浓度的临界值，因此含0.5 mmol/L银电解液制备的材料用于临床更安全可靠。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 缓释材料, 纳米银, 羟基磷灰石, 钛金属, 电泳沉积, 复合涂层, 银离子缓释性能, 抗菌活性, 新疆维吾尔自治区自然科学基金

Abstract:

BACKGROUND: Surface modification of orthopedic implants can reduce or prevent bacterial adhersion. Bacteriostatic and bactericidal ingredients released from special coating of metal surfaces prevent orthopedic surgery infection.
OBJECTIVE: To prepare hydroxyapatite/nano-silver composite coating on the surface of medical titanium based on different preparation parameters and to observe the release properties of silver ions on the composite material surface in the simulated body fluid.
METHODS: Using pulse electrochemical methods, hydroxyapatite and nano-silver were deposited in the solution containing silver, calcium and phosphate ions. Scanning electron microscopy, X-ray diffraction and energy dispersive spectroscopy were used to characterize its morphology and composition. The composite titanium materials containing 0.5, 1 mmol/L silver were immersed in the simulated body fluid, and Ag+ concentration was detected by atomic absorption spectrometry at the different time points.
RESULTS AND CONCLUSION: Nanoparticles were uniformly distributed in the coating which was interwoven with the nano needle-like hydroxypatite and dot-like silver particles. After high temperature processing, the coating became denser, and hydroxypatite became more crystal and silver particles exhibited no agglomeration. In the simulated body fluid, Ag+ release was maximal at 1-7 days and became stable at 7-30 days which maintained an effective antimicrobial concentration. The material containing 0.5 mmol/L Ag+ showed a lower amount of Ag+ released than cytotoxic concentration at 30 days, but the material containing 1 mmol/L Ag+ could release the total of Ag+ close to the critical value of cell toxicity at 30 days. Above all, the material containing 0.5 mmol/L Ag+ is more secure in the clinical application.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: hydroxyapatites, metal nanoparticles, titanium

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